Oct 2018 - 2nd Instruct Workshop on Cryo-EM Best Practices

NeCEN organised the second Instruct workshop on cryo-EM best practices on 15-16 Oct 2018


Ludo Renault, Susanne Roodhuyzen




To share best practices between cryoEM centres for the delivery of services to academia and companies.

Following on the success of the first Instruct Workshop on cryo-EM best practices held in Harwell in May 2017 (link), NeCEN organised the second version that took place in Leiden, The Netherlands on 15-16 Oct 2018.

Focus was given towards operations, remote control, efficient usage of the machines and live processing in two separate sessions followed by round table discussions.

Recordings of the presentations and notes from the round table discussions can be found on this page. The full program is available here.

This workshop is part of the Instruct-ULTRA training program / Instruct-ERIC.


Session 1: Operations and ensuring efficiency of access

An update on eBIC - Electron Bio-Imaging Centre (eBIC), Diamond Light Source, UK (Daniel Clare)



Swedish National CryoEM facility: combining screening and data collection for internal and external users - SciLifeLab, Stockholm, Sweden (Marta Carroni)



Cryo-EM at Leeds- approaches and challenges for high resolution data acquisition – Astbury Centre, Leeds, UK (Rebecca Thompson)



Unified EM setup: the benefits of SerialEM for facilities – EMBL, Heidelberg, Germany (Wim Hagen)



Discussion 1 (chairs: Marta Carroni & Ludo Renault)

Topics: Remote viewing, Remote control, Ensuring efficient usage of the machines



Plenary Session

Current status and perspectives of Cryo-EM in Brazil – LNNano, Campinas, Brazil (Rodrigo Portugal)


Cryo-EM at the European Synchrotron - Current status and future developments - European Synchrotron Radiation Facility, Grenoble, France (Christoph Mueller-Dieckmann)



Session 2: Data management and live processing

Pipeline data collection at the IGBMC cryo-EM platform –IGBMC, Illkrich, France (Ottilie von Loeffelholz )



Scipion for facilities – CNB-CSIC, Madrid, Spain (Pablo Conesa)



System for high-throughput cryo-EM at atomic resolution - Tsinghua University, Beijing, China (Xueming Li)


Cryo-EM data pre-processing at full Warp – MPI-BPC Gottingen, Germany (Dimitry Tegunov)



Discussion 2 (chairs: Pablo Conesa & Ludo Renault)

Topics: Data Management, Live processing, Deliverables