Cryo-EM School

2018 Cryo-EM School - Full!

The 2018 Fall Cryo-EM School will take place at The Netherlands Centre for Electron Nanoscopy (NeCEN). From the 22nd of October until the 16th of November, 6 participants will receive hands-on Titan Krios training from the experienced NeCEN EM scientists and Thermo Fisher applications support team. The possibility to bring your own sample exists.


Part of the Cryo-EM School is a 3-day Image Processing Course organised in collaboration with the Biocomputing Unit of the Spanish National Center for Biotechnology (CNB).

Location

NeCEN, Leiden University, The Netherlands

Partners

  • Thermo Fisher Scientific
  • The Netherlands Centre for Electron Nanoscopy (NeCEN)
  • The Biocomputing Unit of the Spanish National Center for Biotechnology (CNB)

Curriculum

The curriculum is divided in two parts.

  1. Online lectures about the theory of cryo-EM single particle analysis as well as practical demonstrations by Grant Jensen (CalTech) and Matthijn Vos (Thermo Fisher Scientific) (http://em-learning.com). The school participants have to follow the online course and take the self-evaluation test before coming to NeCEN for practical hands-on training.
  2. Hands-on practicals on the different aspects taught online.
  • Cryo-EM sample freezing on a vitrobot
  • Cryo-EM grids clipping and loading for autoloader systems
  • Cryo-EM sample screening
  • Titan Krios alignments
  • Automated Single Particle Data Acquisition with EPU
  • Basics of image processing within Scipion Environment in the cloud

Participation

If you are interested in participating, please contact Matthijn Vos at matthijn.vos@fei.com

The course gives you 4 weeks access to 2 Krios microscopes equipped with Falcon 3EC and K2 Summit cameras, 2 dedicated expert teachers, housing with breakfast and lunch during the course days as well as transportation on site. Not included is travel to Leiden and dinners. The non-profit costs are ~30.000 euro or an equivalent of 30 days pre-purchased Thermo Fisher Scientific on-site application support. 

More information

For more information, please contact Susanne Roodhuyzen at S.A.Roodhuijzen@biology.leidenuniv.nl.