1.12 Å ApoF Benchmark
To test performance at high magnification, we collected an overnight single-particle dataset on apoferritin (ApoF). Standard processing in cryoSPARC yielded a reconstruction from 400,000 particles at 1.12 Šresolution (gold-standard FSC = 0.143 cutoff; B-factor of 20.3 Ų).
At 1.12 Å, aromatic ring holes and carbonyl oxygens are resolved, protrusions corresponding to hydrogen atoms are visible in the density, and high-threshold contouring isolates individual atomic peaks. The benchmark confirms that the new Krios 5 in Leiden is ready for user collections across both academic and industrial projects.
User Access
The Krios 5 is open to both academic and industrial researchers.
To discuss sample feasibility, access routes, or beamtime scheduling, please contact our team at necen@biology.leidenuniv.nl
Download the 1.12 Å ApoF cryo-EM map and fitted atomic coordinates
password: necennecen