Application form Do you want access to our facilities? Please use our data collection application form. Fields with * are required.User contact informationFirst name *Infix (e.g. van der)Last name *Form of address (title)Organisation *Department *Organisation address *Telephone number *Mobile phoneE-mail address scientific contact *E-mail address financial department *VAT number (mandatory for EU users, otherwise 21% Dutch VAT) *(mandatory for EU users, otherwise 21% Dutch VAT)Reference number (e.g. SAP number)(e.g. SAP number)FedEx number (or other shipping company) *(e.g. Fedex number)Do you have a Titan Krios Microscope in your institution? * No Yes Specify billing address (different from above) Specify shipping address (different from above)Billing addressName organisationAddress (Street, Postal Code, City, Country)E-mail address *TelephoneContact person *Shipping addressName organisationAddress (Street, Postal Code, City, Country)E-mail address *TelephoneContact person *NeCEN data collection project informationTitle of project *Scientific background(general introduction, ½ page) *Brief description of the project(½-1 page, please include in de description of the project the current results and, if available, some details on the current image reconstruction you have available that support the need for high resolution data collection) *Expected outcome of the data to be collected(Please state in one sentence the main research question and the research question the data could answer) *Relevant publications (maximum 5)Additional information(please state name here if applicable and attach file to email)Negative stain 2D imageFilesize should be less than 2MBCryo-EM 2D imageFilesize should be less than 2MB3D modelFilesize should be less than 2MBBiohazards * No Yes, please specify:Specification biohazardMicroscope informationTechnique * Single particle acquisition Tomography Other, please specify:Specification techniqueAt NeCEN, there are two Titan Krios instruments available dedicated to high-end data collection. Titan Krios 1 is a dedicated instrument for EFTEM (energy-filtered TEM) and cryo-electron tomography. This microscope has a S-FEG source and is equipped with a Gatan GIF+K2 DED as well as a volta phase plate. It is an instrument for both tomography and automated single particle acquisition. Titan Krios 2 is a specialised instrument for single particle acquisition. This microscope has a X-FEG source, a Cs corrector, a Falcon 3 counting mode DED camera and volta phase plate.Accelerating voltage * 80 keV 200 keV 300 keVPlease indicate in the description of the project why you opt for the voltage selected.Requested microscope time * 1 day 2 days 3 days 4 days Other, please specify:Specification timePlease indicate in the description of the project why you opt for this number of days. Please note that the data will be collected by the NeCEN operator accompanied by, if needed, the applicant.Required specimen preparation * None Plunge freezing Other, please specify:If specimens are not prepared, they will be prepared at the home institution, prior to the NeCEN visit.Specification preparation