Netherlands Centre for Electron Nanoscopy

Cryo tomographic studies

At present cryo tomography is performed on either thin biological specimens or on cryo sections of high pressure frozen samples. To overcome the limitations of cryo-sectioning Dr. Jan Andries Post (Utrecht University) wants to employ cryo Focused Ion Beam – Scanning Electron Microscopy to prepare thicker cryo sections from High Pressure Freezer (HPF) specimens. Studying the obtained specimens with the NeCEN facilities will lead to a wealth of information on the 3D architecture of cells and new insights in cellular functioning.

Publication

Hayles, M. F., D. A. Matthijs de Winter, C. T. W. M. Schneijdenberg, J. D. Meeldijk, U. Luecken, H. Persoon, J. de Water, F. de Jong, B. M. Humbel and A. J. Verkleij “The making of frozen-hydrated, vitreous lamellas from cells for cryo-electron microscopy.” Journal of Structural Biology 172(2): 180-190